Detailed information of TA system TA283669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93656..94082
• Replicon: plasmid pKPC2_130128
• Accession: NZ_CP127236
• Organism: Klebsiella pneumoniae strain 130128

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QRA17_RS27555
• Protein ID: WP_001372321.1
• Coordinates: 93656..93781 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93858..94082 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QRA17_RS27520 (88711)	88711..88938	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
QRA17_RS27525 (89032)	89032..89718	-	687	WP_015059009.1	PAS domain-containing protein	-
QRA17_RS27530 (89909)	89909..90292	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QRA17_RS27535 (90569)	90569..91216	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
QRA17_RS27540 (91513)	91513..92334	-	822	WP_001234445.1	DUF932 domain-containing protein	-
QRA17_RS27545 (92445)	92445..92741	-	297	WP_001272251.1	hypothetical protein	-
QRA17_RS27550 (93041)	93041..93337	+	297	Protein_121	hypothetical protein	-
QRA17_RS27555 (93656)	93656..93781	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QRA17_RS27560 (93723)	93723..93872	-	150	Protein_123	plasmid maintenance protein Mok	-
- (93858)	93858..94082	-	225	NuclAT_0	-	Antitoxin
- (93858)	93858..94082	-	225	NuclAT_0	-	Antitoxin
- (93858)	93858..94082	-	225	NuclAT_0	-	Antitoxin
- (93858)	93858..94082	-	225	NuclAT_0	-	Antitoxin
QRA17_RS27565 (94094)	94094..94813	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
QRA17_RS27570 (94810)	94810..95244	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
QRA17_RS27575 (95313)	95313..97336	-	2024	Protein_126	ParB/RepB/Spo0J family partition protein	-
QRA17_RS27580 (97397)	97397..97630	-	234	WP_000006003.1	DUF905 family protein	-
QRA17_RS27585 (97688)	97688..98215	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
QRA17_RS27590 (98517)	98517..98978	+	462	Protein_129	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..154719	154719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T283669 WP_001372321.1 NZ_CP127236:c93781-93656 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT283669 NZ_CP127236:c94082-93858 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin