Detailed information of TA system TA283646

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50056..50482
• Replicon: plasmid pKPC2_130119
• Accession: NZ_CP127230
• Organism: Klebsiella pneumoniae strain 130119

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QRA14_RS27010
• Protein ID: WP_001372321.1
• Coordinates: 50357..50482 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50056..50280 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QRA14_RS26975 (45166)	45166..45621	-	456	Protein_57	hypothetical protein	-
QRA14_RS26980 (45923)	45923..46450	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
QRA14_RS26985 (46508)	46508..46741	+	234	WP_000006003.1	DUF905 family protein	-
QRA14_RS26990 (46802)	46802..48825	+	2024	Protein_60	ParB/RepB/Spo0J family partition protein	-
QRA14_RS26995 (48894)	48894..49328	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
QRA14_RS27000 (49325)	49325..50044	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
- (50056)	50056..50280	+	225	NuclAT_0	-	Antitoxin
- (50056)	50056..50280	+	225	NuclAT_0	-	Antitoxin
- (50056)	50056..50280	+	225	NuclAT_0	-	Antitoxin
- (50056)	50056..50280	+	225	NuclAT_0	-	Antitoxin
QRA14_RS27005 (50266)	50266..50415	+	150	Protein_63	plasmid maintenance protein Mok	-
QRA14_RS27010 (50357)	50357..50482	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QRA14_RS27015 (50801)	50801..51097	-	297	Protein_65	hypothetical protein	-
QRA14_RS27020 (51397)	51397..51693	+	297	WP_001272251.1	hypothetical protein	-
QRA14_RS27025 (51804)	51804..52625	+	822	WP_001234445.1	DUF932 domain-containing protein	-
QRA14_RS27030 (52922)	52922..53569	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
QRA14_RS27035 (53846)	53846..54229	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QRA14_RS27040 (54420)	54420..55106	+	687	WP_015059009.1	PAS domain-containing protein	-
QRA14_RS27045 (55200)	55200..55427	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..153000	153000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T283646 WP_001372321.1 NZ_CP127230:50357-50482 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT283646 NZ_CP127230:50056-50280 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin