Detailed information of TA system TA028358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5110772..5111374
• Replicon: chromosome
• Accession: NC_018661
• Organism: Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: O3O_RS25860
• Protein ID: WP_000897305.1
• Coordinates: 5111063..5111374 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: O3O_RS25855
• Protein ID: WP_000356397.1
• Coordinates: 5110772..5111062 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3O_RS25825	5106716..5107618	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
O3O_RS25830	5107615..5108250	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
O3O_RS25835	5108247..5109176	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
O3O_RS25845	5109506..5109748	-	243	WP_001087409.1	hypothetical protein	-
O3O_RS25850	5109968..5110186	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
O3O_RS25855	5110772..5111062	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
O3O_RS25860	5111063..5111374	-	312	WP_000897305.1	hypothetical protein	Toxin
O3O_RS25865	5111603..5112511	+	909	WP_001387201.1	alpha/beta hydrolase	-
O3O_RS25870	5112575..5113516	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
O3O_RS25875	5113561..5113998	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
O3O_RS25880	5113995..5114867	-	873	WP_000920762.1	virulence factor BrkB family protein	-
O3O_RS25885	5114861..5115460	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T28358 WP_000897305.1 NC_018661:c5111374-5111063 [Escherichia coli O104:H4 str. 2009EL-2071]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T28358 NC_018661:c5111374-5111063 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT28358 WP_000356397.1 NC_018661:c5111062-5110772 [Escherichia coli O104:H4 str. 2009EL-2071]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT28358 NC_018661:c5111062-5110772 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7