Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 320124..320719 Replicon chromosome
Accession NZ_CP126965
Organism Micrococcus lylae strain UMB0955

Toxin (Protein)


Gene name graT Uniprot ID A0A1R4IY77
Locus tag CJ228_RS01430 Protein ID WP_067189890.1
Coordinates 320124..320384 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1R4IY79
Locus tag CJ228_RS01435 Protein ID WP_067189892.1
Coordinates 320399..320719 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ228_RS01420 (CJ228_001420) 316287..318857 + 2571 WP_102214445.1 PEP/pyruvate-binding domain-containing protein -
CJ228_RS01425 (CJ228_001425) 318969..319898 + 930 WP_067189888.1 diaminopimelate epimerase -
CJ228_RS01430 (CJ228_001430) 320124..320384 + 261 WP_067189890.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CJ228_RS01435 (CJ228_001435) 320399..320719 + 321 WP_067189892.1 HigA family addiction module antitoxin Antitoxin
CJ228_RS01440 (CJ228_001440) 320853..321488 - 636 WP_102214501.1 methyltransferase -
CJ228_RS01445 (CJ228_001445) 321901..323520 + 1620 WP_088478010.1 GTPase HflX -
CJ228_RS01450 (CJ228_001450) 323520..325604 + 2085 WP_102214443.1 ATP-dependent DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 307383..359564 52181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-80)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(14-103)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10177.80 Da        Isoelectric Point: 11.3217

>T283410 WP_067189890.1 NZ_CP126965:320124-320384 [Micrococcus lylae]
MIRSFGSKDTERIWHGAYVKRIDRTVQRATLRKLEMINAAHVVDDLRIPPGNRLEQLVGDRRGQYSIRVNTQWRLCFVWT
ALLRLD

Download         Length: 261 bp

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11919.61 Da        Isoelectric Point: 4.5837

>AT283410 WP_067189892.1 NZ_CP126965:320399-320719 [Micrococcus lylae]
MSNSSTATDLDLIEPVHPGEILLADFIQGFDLSQNRVAVALGVPPRRINEIVLGKRGITADTAIRLARYFGTSEQFWMNL
QVDYELRMQHRVLDGQLEEIQPLQVA

Download         Length: 321 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1R4IY77

Antitoxin

Source ID Structure
AlphaFold DB A0A1R4IY79

References