Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1960513..1961015 Replicon chromosome
Accession NC_018661
Organism Escherichia coli O104:H4 str. 2009EL-2071

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag O3O_RS09655 Protein ID WP_000767829.1
Coordinates 1960761..1961015 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag O3O_RS09650 Protein ID WP_001259255.1
Coordinates 1960513..1960764 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3O_RS09630 1955690..1956757 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
O3O_RS09635 1956757..1957827 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
O3O_RS09640 1957824..1959128 - 1305 WP_001386882.1 histidinol dehydrogenase -
O3O_RS09645 1959134..1960033 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
O3O_RS28330 1960179..1960229 - 51 WP_001364200.1 his operon leader peptide -
O3O_RS09650 1960513..1960764 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
O3O_RS09655 1960761..1961015 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
O3O_RS09660 1961098..1961922 + 825 WP_000754758.1 SDR family oxidoreductase -
O3O_RS09665 1961968..1962897 + 930 WP_000803366.1 LysR family transcriptional regulator -
O3O_RS30940 1963112..1963174 + 63 WP_010723108.1 membrane protein YoeI -
O3O_RS09670 1963164..1964522 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
O3O_RS09675 1964701..1965759 + 1059 WP_000492324.1 transport protein YeeE -
O3O_RS09680 1965773..1966000 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T28340 WP_000767829.1 NC_018661:1960761-1961015 [Escherichia coli O104:H4 str. 2009EL-2071]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T28340 NC_018661:1960761-1961015 [Escherichia coli O104:H4 str. 2009EL-2071]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT28340 WP_001259255.1 NC_018661:1960513-1960764 [Escherichia coli O104:H4 str. 2009EL-2071]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT28340 NC_018661:1960513-1960764 [Escherichia coli O104:H4 str. 2009EL-2071]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References