Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3651286..3651965 Replicon chromosome
Accession NZ_CP126952
Organism Escherichia coli O78:H4 strain APEC E12049

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QQA22_RS18010 Protein ID WP_000057523.1
Coordinates 3651663..3651965 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA22_RS18005 Protein ID WP_000806442.1
Coordinates 3651286..3651627 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA22_RS17995 (3647529) 3647529..3648461 - 933 WP_000883024.1 glutaminase A -
QQA22_RS18000 (3648724) 3648724..3651228 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
QQA22_RS18005 (3651286) 3651286..3651627 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA22_RS18010 (3651663) 3651663..3651965 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA22_RS18015 (3652098) 3652098..3652892 + 795 WP_000365161.1 TraB/GumN family protein -
QQA22_RS18020 (3653096) 3653096..3653575 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA22_RS18025 (3653612) 3653612..3655264 - 1653 WP_039004121.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QQA22_RS18030 (3655482) 3655482..3656702 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3643172..3651965 8793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T283352 WP_000057523.1 NZ_CP126952:c3651965-3651663 [Escherichia coli O78:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283352 WP_000806442.1 NZ_CP126952:c3651627-3651286 [Escherichia coli O78:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References