Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 686142..686941 Replicon chromosome
Accession NZ_CP126952
Organism Escherichia coli O78:H4 strain APEC E12049

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag QQA22_RS03400 Protein ID WP_000347251.1
Coordinates 686142..686606 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag QQA22_RS03405 Protein ID WP_001296435.1
Coordinates 686606..686941 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA22_RS03370 (681142) 681142..681576 - 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QQA22_RS03375 (681594) 681594..682472 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QQA22_RS03380 (682462) 682462..683241 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QQA22_RS03385 (683252) 683252..683725 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QQA22_RS03390 (683748) 683748..685029 - 1282 Protein_664 tagatose-bisphosphate aldolase subunit KbaZ -
QQA22_RS03395 (685278) 685278..686087 + 810 WP_264097422.1 aga operon transcriptional regulator AgaR -
QQA22_RS03400 (686142) 686142..686606 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QQA22_RS03405 (686606) 686606..686941 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QQA22_RS03410 (687090) 687090..688661 - 1572 WP_001273941.1 galactarate dehydratase -
QQA22_RS03415 (689036) 689036..690370 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
QQA22_RS03420 (690386) 690386..691156 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
QQA22_RS03425 (691186) 691186..691449 + 264 Protein_671 NAD(P)-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 691462..692670 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T283339 WP_000347251.1 NZ_CP126952:c686606-686142 [Escherichia coli O78:H4]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT283339 WP_001296435.1 NZ_CP126952:c686941-686606 [Escherichia coli O78:H4]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References