Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3591625..3592304 Replicon chromosome
Accession NZ_CP126948
Organism Escherichia coli O78:H4 strain APEC E14033

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QQA23_RS17745 Protein ID WP_000057523.1
Coordinates 3592002..3592304 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA23_RS17740 Protein ID WP_000806442.1
Coordinates 3591625..3591966 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA23_RS17730 (3587868) 3587868..3588800 - 933 WP_000883024.1 glutaminase A -
QQA23_RS17735 (3589063) 3589063..3591567 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
QQA23_RS17740 (3591625) 3591625..3591966 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA23_RS17745 (3592002) 3592002..3592304 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA23_RS17750 (3592437) 3592437..3593231 + 795 WP_000365161.1 TraB/GumN family protein -
QQA23_RS17755 (3593435) 3593435..3593914 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA23_RS17760 (3593951) 3593951..3595603 - 1653 WP_059338785.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QQA23_RS17765 (3595821) 3595821..3597041 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T283321 WP_000057523.1 NZ_CP126948:c3592304-3592002 [Escherichia coli O78:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283321 WP_000806442.1 NZ_CP126948:c3591966-3591625 [Escherichia coli O78:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References