Detailed information of TA system TA028329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5071207..5071809
• Replicon: chromosome
• Accession: NC_018658
• Organism: Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: O3K_RS24645
• Protein ID: WP_000897305.1
• Coordinates: 5071498..5071809 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: O3K_RS24640
• Protein ID: WP_000356397.1
• Coordinates: 5071207..5071497 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O3K_RS24615	5067151..5068053	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
O3K_RS24620	5068050..5068685	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
O3K_RS24625	5068682..5069611	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
O3K_RS24630	5069941..5070183	-	243	WP_001087409.1	hypothetical protein	-
O3K_RS24635	5070403..5070621	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
O3K_RS24640	5071207..5071497	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
O3K_RS24645	5071498..5071809	-	312	WP_000897305.1	hypothetical protein	Toxin
O3K_RS24650	5072038..5072946	+	909	WP_001387201.1	alpha/beta hydrolase	-
O3K_RS24655	5073010..5073951	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
O3K_RS24660	5073996..5074433	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
O3K_RS24665	5074430..5075302	-	873	WP_000920762.1	virulence factor BrkB family protein	-
O3K_RS24670	5075296..5075895	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T28329 WP_000897305.1 NC_018658:c5071809-5071498 [Escherichia coli O104:H4 str. 2011C-3493]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T28329 NC_018658:c5071809-5071498 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT28329 WP_000356397.1 NC_018658:c5071497-5071207 [Escherichia coli O104:H4 str. 2011C-3493]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT28329 NC_018658:c5071497-5071207 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7