Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4036761..4037560 Replicon chromosome
Accession NZ_CP126946
Organism Escherichia coli O78:H51 strain APEC E18005

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag QQA24_RS19625 Protein ID WP_000347266.1
Coordinates 4037096..4037560 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QQA24_RS19620 Protein ID WP_001307405.1
Coordinates 4036761..4037096 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA24_RS19605 (4032546) 4032546..4033316 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
QQA24_RS19610 (4033332) 4033332..4034666 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QQA24_RS19615 (4035041) 4035041..4036612 + 1572 WP_001273741.1 galactarate dehydratase -
QQA24_RS19620 (4036761) 4036761..4037096 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QQA24_RS19625 (4037096) 4037096..4037560 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QQA24_RS19630 (4037615) 4037615..4038424 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QQA24_RS19635 (4038673) 4038673..4039953 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
QQA24_RS19640 (4039976) 4039976..4040449 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QQA24_RS19645 (4040460) 4040460..4041239 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QQA24_RS19650 (4041229) 4041229..4042107 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
QQA24_RS19655 (4042125) 4042125..4042559 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4025773..4037560 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T283288 WP_000347266.1 NZ_CP126946:4037096-4037560 [Escherichia coli O78:H51]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT283288 WP_001307405.1 NZ_CP126946:4036761-4037096 [Escherichia coli O78:H51]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References