Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4172675..4173216 Replicon chromosome
Accession NC_018658
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag O3K_RS20490 Protein ID WP_000615974.1
Coordinates 4172938..4173216 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag O3K_RS20485 Protein ID WP_000729704.1
Coordinates 4172675..4172935 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS20460 4168458..4169243 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
O3K_RS20465 4169215..4170927 + 1713 Protein_4012 flagellar biosynthesis protein FlhA -
O3K_RS20470 4171032..4171310 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
O3K_RS20475 4171303..4171659 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
O3K_RS20480 4171716..4172489 - 774 WP_000543895.1 C40 family peptidase -
O3K_RS20485 4172675..4172935 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
O3K_RS20490 4172938..4173216 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
O3K_RS20495 4173372..4174112 + 741 WP_001225679.1 murein L,D-transpeptidase -
O3K_RS20500 4174083..4174850 - 768 WP_000333380.1 class II glutamine amidotransferase -
O3K_RS20505 4175056..4175634 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T28325 WP_000615974.1 NC_018658:4172938-4173216 [Escherichia coli O104:H4 str. 2011C-3493]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T28325 NC_018658:4172938-4173216 [Escherichia coli O104:H4 str. 2011C-3493]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT28325 WP_000729704.1 NC_018658:4172675-4172935 [Escherichia coli O104:H4 str. 2011C-3493]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT28325 NC_018658:4172675-4172935 [Escherichia coli O104:H4 str. 2011C-3493]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References