Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3905884..3906578 Replicon chromosome
Accession NZ_CP126944
Organism Escherichia coli O1:K1:H7 strain APEC E16002

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA28_RS19385 Protein ID WP_001263500.1
Coordinates 3905884..3906282 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA28_RS19390 Protein ID WP_000554758.1
Coordinates 3906285..3906578 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA28_RS19360 (3901249) 3901249..3901707 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QQA28_RS19365 (3901968) 3901968..3903425 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA28_RS19370 (3903482) 3903482..3904096 - 615 WP_000602123.1 peptide chain release factor H -
QQA28_RS19375 (3904093) 3904093..3905232 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA28_RS19380 (3905422) 3905422..3905874 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA28_RS19385 (3905884) 3905884..3906282 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA28_RS19390 (3906285) 3906285..3906578 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA28_RS19395 (3906630) 3906630..3907685 - 1056 WP_001226168.1 DNA polymerase IV -
QQA28_RS19400 (3907756) 3907756..3908541 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA28_RS19405 (3908513) 3908513..3910225 + 1713 Protein_3805 flagellar biosynthesis protein FlhA -
QQA28_RS19410 (3910304) 3910304..3910462 + 159 WP_014639450.1 hypothetical protein -
QQA28_RS19415 (3910545) 3910545..3911042 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3905884..3926238 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283247 WP_001263500.1 NZ_CP126944:c3906282-3905884 [Escherichia coli O1:K1:H7]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283247 WP_000554758.1 NZ_CP126944:c3906578-3906285 [Escherichia coli O1:K1:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References