Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3905394..3906088 Replicon chromosome
Accession NZ_CP126942
Organism Escherichia coli O1:K1:H7 strain APEC E18006

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA29_RS19380 Protein ID WP_001263500.1
Coordinates 3905394..3905792 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA29_RS19385 Protein ID WP_000554758.1
Coordinates 3905795..3906088 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA29_RS19355 (3900759) 3900759..3901217 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QQA29_RS19360 (3901478) 3901478..3902935 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA29_RS19365 (3902992) 3902992..3903606 - 615 WP_000602123.1 peptide chain release factor H -
QQA29_RS19370 (3903603) 3903603..3904742 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA29_RS19375 (3904932) 3904932..3905384 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA29_RS19380 (3905394) 3905394..3905792 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA29_RS19385 (3905795) 3905795..3906088 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA29_RS19390 (3906140) 3906140..3907195 - 1056 WP_001226168.1 DNA polymerase IV -
QQA29_RS19395 (3907266) 3907266..3908051 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA29_RS19400 (3908023) 3908023..3909735 + 1713 Protein_3804 flagellar biosynthesis protein FlhA -
QQA29_RS19405 (3909814) 3909814..3909972 + 159 WP_014639450.1 hypothetical protein -
QQA29_RS19410 (3910055) 3910055..3910552 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3905394..3925748 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283212 WP_001263500.1 NZ_CP126942:c3905792-3905394 [Escherichia coli O1:K1:H7]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283212 WP_000554758.1 NZ_CP126942:c3906088-3905795 [Escherichia coli O1:K1:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References