Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3951205..3951899 Replicon chromosome
Accession NZ_CP126940
Organism Escherichia coli O2:K1:H7 strain APEC E18055

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA30_RS19635 Protein ID WP_001263500.1
Coordinates 3951205..3951603 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA30_RS19640 Protein ID WP_000554758.1
Coordinates 3951606..3951899 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA30_RS19610 (3946570) 3946570..3947028 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QQA30_RS19615 (3947289) 3947289..3948746 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA30_RS19620 (3948803) 3948803..3949417 - 615 WP_231528506.1 peptide chain release factor H -
QQA30_RS19625 (3949414) 3949414..3950553 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA30_RS19630 (3950743) 3950743..3951195 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA30_RS19635 (3951205) 3951205..3951603 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA30_RS19640 (3951606) 3951606..3951899 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA30_RS19645 (3951951) 3951951..3953006 - 1056 WP_001226168.1 DNA polymerase IV -
QQA30_RS19650 (3953077) 3953077..3953862 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA30_RS19655 (3953834) 3953834..3955546 + 1713 Protein_3853 flagellar biosynthesis protein FlhA -
QQA30_RS19660 (3955625) 3955625..3955783 + 159 WP_014639450.1 hypothetical protein -
QQA30_RS19665 (3955866) 3955866..3956363 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3951205..3971559 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283183 WP_001263500.1 NZ_CP126940:c3951603-3951205 [Escherichia coli O2:K1:H7]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283183 WP_000554758.1 NZ_CP126940:c3951899-3951606 [Escherichia coli O2:K1:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References