Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3709631..3710310 Replicon chromosome
Accession NZ_CP126940
Organism Escherichia coli O2:K1:H7 strain APEC E18055

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag QQA30_RS18490 Protein ID WP_000057524.1
Coordinates 3710008..3710310 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA30_RS18485 Protein ID WP_000806442.1
Coordinates 3709631..3709972 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA30_RS18475 (3705875) 3705875..3706807 - 933 WP_000883052.1 glutaminase A -
QQA30_RS18480 (3707069) 3707069..3709573 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
QQA30_RS18485 (3709631) 3709631..3709972 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA30_RS18490 (3710008) 3710008..3710310 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA30_RS18495 (3710443) 3710443..3711237 + 795 WP_000365177.1 TraB/GumN family protein -
QQA30_RS18500 (3711441) 3711441..3711920 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA30_RS18505 (3711944) 3711944..3712744 + 801 WP_000439796.1 hypothetical protein -
QQA30_RS18510 (3712741) 3712741..3713244 + 504 WP_000667000.1 hypothetical protein -
QQA30_RS18515 (3713282) 3713282..3714934 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3702320..3713244 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T283181 WP_000057524.1 NZ_CP126940:c3710310-3710008 [Escherichia coli O2:K1:H7]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283181 WP_000806442.1 NZ_CP126940:c3709972-3709631 [Escherichia coli O2:K1:H7]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References