Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2662836..2663398 Replicon chromosome
Accession NZ_CP126940
Organism Escherichia coli O2:K1:H7 strain APEC E18055

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QQA30_RS13125 Protein ID WP_000605675.1
Coordinates 2662836..2663114 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QQA30_RS13130 Protein ID WP_000781370.1
Coordinates 2663114..2663398 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA30_RS13100 (2658423) 2658423..2658854 - 432 WP_000152310.1 peroxiredoxin OsmC -
QQA30_RS13105 (2659199) 2659199..2659414 + 216 WP_000495766.1 biofilm-dependent modulation protein -
QQA30_RS13110 (2659516) 2659516..2659653 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QQA30_RS13115 (2659809) 2659809..2661506 + 1698 WP_000433462.1 malate dehydrogenase -
QQA30_RS13120 (2661640) 2661640..2662650 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QQA30_RS13125 (2662836) 2662836..2663114 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA30_RS13130 (2663114) 2663114..2663398 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QQA30_RS13135 (2663627) 2663627..2664280 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
QQA30_RS13140 (2664273) 2664273..2665157 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
QQA30_RS13145 (2665170) 2665170..2668217 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T283180 WP_000605675.1 NZ_CP126940:2662836-2663114 [Escherichia coli O2:K1:H7]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T283180 NZ_CP126940:2662836-2663114 [Escherichia coli O2:K1:H7]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT283180 WP_000781370.1 NZ_CP126940:2663114-2663398 [Escherichia coli O2:K1:H7]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT283180 NZ_CP126940:2663114-2663398 [Escherichia coli O2:K1:H7]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References