Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3761106..3761800 Replicon chromosome
Accession NZ_CP126937
Organism Escherichia coli O2:K1:H4 strain APEC E19019

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA31_RS18405 Protein ID WP_001263500.1
Coordinates 3761106..3761504 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA31_RS18410 Protein ID WP_000554758.1
Coordinates 3761507..3761800 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA31_RS18380 (3756471) 3756471..3756929 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QQA31_RS18385 (3757190) 3757190..3758647 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA31_RS18390 (3758704) 3758704..3759318 - 615 WP_000602123.1 peptide chain release factor H -
QQA31_RS18395 (3759315) 3759315..3760454 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA31_RS18400 (3760644) 3760644..3761096 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA31_RS18405 (3761106) 3761106..3761504 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA31_RS18410 (3761507) 3761507..3761800 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA31_RS18415 (3761852) 3761852..3762907 - 1056 WP_001226168.1 DNA polymerase IV -
QQA31_RS18420 (3762978) 3762978..3763763 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA31_RS18425 (3763735) 3763735..3765447 + 1713 Protein_3611 flagellar biosynthesis protein FlhA -
QQA31_RS18430 (3765526) 3765526..3765684 + 159 WP_014639450.1 hypothetical protein -
QQA31_RS18435 (3765767) 3765767..3766264 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3761106..3781459 20353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283160 WP_001263500.1 NZ_CP126937:c3761504-3761106 [Escherichia coli O2:K1:H4]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283160 WP_000554758.1 NZ_CP126937:c3761800-3761507 [Escherichia coli O2:K1:H4]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References