Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3497124..3497803 Replicon chromosome
Accession NZ_CP126937
Organism Escherichia coli O2:K1:H4 strain APEC E19019

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag QQA31_RS17110 Protein ID WP_000057524.1
Coordinates 3497501..3497803 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA31_RS17105 Protein ID WP_000806442.1
Coordinates 3497124..3497465 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA31_RS17095 (3493368) 3493368..3494300 - 933 WP_000883052.1 glutaminase A -
QQA31_RS17100 (3494562) 3494562..3497066 + 2505 WP_016239198.1 copper-exporting P-type ATPase CopA -
QQA31_RS17105 (3497124) 3497124..3497465 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA31_RS17110 (3497501) 3497501..3497803 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA31_RS17115 (3497936) 3497936..3498730 + 795 WP_000365177.1 TraB/GumN family protein -
QQA31_RS17120 (3498934) 3498934..3499413 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA31_RS17125 (3499437) 3499437..3500237 + 801 WP_000439796.1 hypothetical protein -
QQA31_RS17130 (3500234) 3500234..3500737 + 504 WP_000667000.1 hypothetical protein -
QQA31_RS17135 (3500775) 3500775..3502427 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3489813..3500737 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T283158 WP_000057524.1 NZ_CP126937:c3497803-3497501 [Escherichia coli O2:K1:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283158 WP_000806442.1 NZ_CP126937:c3497465-3497124 [Escherichia coli O2:K1:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References