Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3006216..3007015 Replicon chromosome
Accession NZ_CP126934
Organism Escherichia coli O78:H4 strain APEC E19057

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag QQA26_RS15025 Protein ID WP_000347251.1
Coordinates 3006551..3007015 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QQA26_RS15020 Protein ID WP_001307405.1
Coordinates 3006216..3006551 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA26_RS15005 (3002001) 3002001..3002771 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
QQA26_RS15010 (3002787) 3002787..3004121 - 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
QQA26_RS15015 (3004496) 3004496..3006067 + 1572 WP_001273761.1 galactarate dehydratase -
QQA26_RS15020 (3006216) 3006216..3006551 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QQA26_RS15025 (3006551) 3006551..3007015 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QQA26_RS15030 (3007070) 3007070..3007879 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QQA26_RS15035 (3008128) 3008128..3009408 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
QQA26_RS15040 (3009431) 3009431..3009904 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QQA26_RS15045 (3009915) 3009915..3010694 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QQA26_RS15050 (3010684) 3010684..3011562 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QQA26_RS15055 (3011580) 3011580..3012014 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2995341..3007015 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T283125 WP_000347251.1 NZ_CP126934:3006551-3007015 [Escherichia coli O78:H4]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT283125 WP_001307405.1 NZ_CP126934:3006216-3006551 [Escherichia coli O78:H4]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References