Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1964782..1965284 Replicon chromosome
Accession NC_018658
Organism Escherichia coli O104:H4 str. 2011C-3493

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag O3K_RS09375 Protein ID WP_000767829.1
Coordinates 1965030..1965284 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag O3K_RS09370 Protein ID WP_001259255.1
Coordinates 1964782..1965033 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3K_RS09345 1959959..1961026 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
O3K_RS09350 1961026..1962096 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
O3K_RS09355 1962093..1963397 - 1305 WP_001386882.1 histidinol dehydrogenase -
O3K_RS09360 1963403..1964302 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
O3K_RS09365 1964448..1964498 - 51 WP_001364200.1 his operon leader peptide -
O3K_RS09370 1964782..1965033 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
O3K_RS09375 1965030..1965284 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
O3K_RS09380 1965367..1966191 + 825 WP_000754758.1 SDR family oxidoreductase -
O3K_RS09385 1966237..1967166 + 930 WP_000803366.1 LysR family transcriptional regulator -
O3K_RS09390 1967381..1967443 + 63 WP_010723108.1 membrane protein YoeI -
O3K_RS09395 1967433..1968791 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
O3K_RS09400 1968970..1970028 + 1059 WP_000492324.1 transport protein YeeE -
O3K_RS09405 1970042..1970269 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T28312 WP_000767829.1 NC_018658:1965030-1965284 [Escherichia coli O104:H4 str. 2011C-3493]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T28312 NC_018658:1965030-1965284 [Escherichia coli O104:H4 str. 2011C-3493]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT28312 WP_001259255.1 NC_018658:1964782-1965033 [Escherichia coli O104:H4 str. 2011C-3493]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT28312 NC_018658:1964782-1965033 [Escherichia coli O104:H4 str. 2011C-3493]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References