Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3701295..3701989 Replicon chromosome
Accession NZ_CP126928
Organism Escherichia coli O2:K1:H4 strain APEC E12053

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA27_RS18230 Protein ID WP_001263500.1
Coordinates 3701591..3701989 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA27_RS18225 Protein ID WP_000554758.1
Coordinates 3701295..3701588 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA27_RS18200 (3696858) 3696858..3697328 + 471 Protein_3564 transposase -
QQA27_RS18205 (3697411) 3697411..3697569 - 159 WP_014639450.1 hypothetical protein -
QQA27_RS18210 (3697648) 3697648..3699360 - 1713 Protein_3566 flagellar biosynthesis protein FlhA -
QQA27_RS18215 (3699332) 3699332..3700117 + 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA27_RS18220 (3700188) 3700188..3701243 + 1056 WP_001226168.1 DNA polymerase IV -
QQA27_RS18225 (3701295) 3701295..3701588 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA27_RS18230 (3701591) 3701591..3701989 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA27_RS18235 (3701999) 3701999..3702451 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA27_RS18240 (3702641) 3702641..3703780 + 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA27_RS18245 (3703777) 3703777..3704391 + 615 WP_000602123.1 peptide chain release factor H -
QQA27_RS18250 (3704448) 3704448..3705905 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA27_RS18255 (3706166) 3706166..3706624 + 459 WP_001291988.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283060 WP_001263500.1 NZ_CP126928:3701591-3701989 [Escherichia coli O2:K1:H4]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283060 WP_000554758.1 NZ_CP126928:3701295-3701588 [Escherichia coli O2:K1:H4]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References