Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3529505..3530184 Replicon chromosome
Accession NZ_CP126928
Organism Escherichia coli O2:K1:H4 strain APEC E12053

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag QQA27_RS17405 Protein ID WP_000057524.1
Coordinates 3529882..3530184 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA27_RS17400 Protein ID WP_000806442.1
Coordinates 3529505..3529846 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA27_RS17390 (3525749) 3525749..3526681 - 933 WP_000883052.1 glutaminase A -
QQA27_RS17395 (3526943) 3526943..3529447 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
QQA27_RS17400 (3529505) 3529505..3529846 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA27_RS17405 (3529882) 3529882..3530184 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA27_RS17410 (3530317) 3530317..3531111 + 795 WP_000365177.1 TraB/GumN family protein -
QQA27_RS17415 (3531315) 3531315..3531794 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA27_RS17420 (3531818) 3531818..3532396 + 579 WP_021522671.1 hypothetical protein -
QQA27_RS17425 (3532615) 3532615..3533118 + 504 WP_000667000.1 hypothetical protein -
QQA27_RS17430 (3533156) 3533156..3534808 - 1653 WP_001513633.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3522194..3533118 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T283058 WP_000057524.1 NZ_CP126928:c3530184-3529882 [Escherichia coli O2:K1:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283058 WP_000806442.1 NZ_CP126928:c3529846-3529505 [Escherichia coli O2:K1:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References