Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2396532..2397094 Replicon chromosome
Accession NZ_CP126928
Organism Escherichia coli O2:K1:H4 strain APEC E12053

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QQA27_RS11665 Protein ID WP_000605675.1
Coordinates 2396816..2397094 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QQA27_RS11660 Protein ID WP_000781370.1
Coordinates 2396532..2396816 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA27_RS11645 (2391713) 2391713..2394760 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
QQA27_RS11650 (2394773) 2394773..2395657 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
QQA27_RS11655 (2395650) 2395650..2396303 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
QQA27_RS11660 (2396532) 2396532..2396816 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QQA27_RS11665 (2396816) 2396816..2397094 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA27_RS11670 (2397280) 2397280..2398290 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QQA27_RS11675 (2398424) 2398424..2400121 - 1698 WP_289245902.1 malate dehydrogenase -
QQA27_RS11680 (2400277) 2400277..2400414 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QQA27_RS11685 (2400516) 2400516..2400731 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QQA27_RS11690 (2401076) 2401076..2401507 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T283052 WP_000605675.1 NZ_CP126928:c2397094-2396816 [Escherichia coli O2:K1:H4]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT283052 WP_000781370.1 NZ_CP126928:c2396816-2396532 [Escherichia coli O2:K1:H4]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References