Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3984307..3985001 Replicon chromosome
Accession NZ_CP126926
Organism Escherichia coli O2:K1:H5 strain APEC E19035

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QQA32_RS19600 Protein ID WP_001263500.1
Coordinates 3984307..3984705 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QQA32_RS19605 Protein ID WP_000554758.1
Coordinates 3984708..3985001 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA32_RS19575 (3979672) 3979672..3980130 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QQA32_RS19580 (3980391) 3980391..3981848 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QQA32_RS19585 (3981905) 3981905..3982519 - 615 WP_000602123.1 peptide chain release factor H -
QQA32_RS19590 (3982516) 3982516..3983655 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QQA32_RS19595 (3983845) 3983845..3984297 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QQA32_RS19600 (3984307) 3984307..3984705 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QQA32_RS19605 (3984708) 3984708..3985001 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QQA32_RS19610 (3985053) 3985053..3986108 - 1056 WP_001226168.1 DNA polymerase IV -
QQA32_RS19615 (3986179) 3986179..3986964 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
QQA32_RS19620 (3986936) 3986936..3988648 + 1713 Protein_3847 flagellar biosynthesis protein FlhA -
QQA32_RS19625 (3988727) 3988727..3988885 + 159 WP_014639450.1 hypothetical protein -
QQA32_RS19630 (3988968) 3988968..3989465 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3984307..4004661 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T283029 WP_001263500.1 NZ_CP126926:c3984705-3984307 [Escherichia coli O2:K1:H5]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT283029 WP_000554758.1 NZ_CP126926:c3985001-3984708 [Escherichia coli O2:K1:H5]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References