Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3755390..3756069 Replicon chromosome
Accession NZ_CP126926
Organism Escherichia coli O2:K1:H5 strain APEC E19035

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag QQA32_RS18540 Protein ID WP_000057524.1
Coordinates 3755767..3756069 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQA32_RS18535 Protein ID WP_000806442.1
Coordinates 3755390..3755731 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQA32_RS18525 (3751634) 3751634..3752566 - 933 WP_000883052.1 glutaminase A -
QQA32_RS18530 (3752828) 3752828..3755332 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
QQA32_RS18535 (3755390) 3755390..3755731 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQA32_RS18540 (3755767) 3755767..3756069 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQA32_RS18545 (3756202) 3756202..3756996 + 795 WP_000365177.1 TraB/GumN family protein -
QQA32_RS18550 (3757200) 3757200..3757679 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQA32_RS18555 (3757703) 3757703..3758503 + 801 WP_000439798.1 hypothetical protein -
QQA32_RS18560 (3758500) 3758500..3759003 + 504 WP_000667000.1 hypothetical protein -
QQA32_RS18565 (3759041) 3759041..3760692 - 1652 Protein_3638 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3748079..3759003 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T283027 WP_000057524.1 NZ_CP126926:c3756069-3755767 [Escherichia coli O2:K1:H5]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283027 WP_000806442.1 NZ_CP126926:c3755731-3755390 [Escherichia coli O2:K1:H5]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References