Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 437023..437689 Replicon chromosome
Accession NZ_CP126536
Organism Citrobacter freundii strain CRNMS1

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag QOK75_RS02085 Protein ID WP_003847996.1
Coordinates 437372..437689 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag QOK75_RS02080 Protein ID WP_003837894.1
Coordinates 437023..437319 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOK75_RS02065 (434199) 434199..434672 - 474 WP_003023524.1 transcription elongation factor GreB -
QOK75_RS02070 (434898) 434898..435617 + 720 WP_001157751.1 two-component system response regulator OmpR -
QOK75_RS02075 (435614) 435614..436966 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
QOK75_RS02080 (437023) 437023..437319 - 297 WP_003837894.1 NadS family protein Antitoxin
QOK75_RS02085 (437372) 437372..437689 - 318 WP_003847996.1 hypothetical protein Toxin
QOK75_RS02090 (437812) 437812..439434 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
QOK75_RS02095 (439813) 439813..441531 + 1719 WP_134214941.1 DUF4153 domain-containing protein -
QOK75_RS02100 (441636) 441636..442514 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T282939 WP_003847996.1 NZ_CP126536:c437689-437372 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT282939 WP_003837894.1 NZ_CP126536:c437319-437023 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References