Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4414191..4414793 Replicon chromosome
Accession NZ_CP126348
Organism Escherichia coli strain NN5

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag QN227_RS21060 Protein ID WP_000897305.1
Coordinates 4414482..4414793 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QN227_RS21055 Protein ID WP_000356397.1
Coordinates 4414191..4414481 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN227_RS21030 (4409757) 4409757..4410392 + 636 WP_000829008.1 formate dehydrogenase cytochrome b556 subunit -
QN227_RS21035 (4410389) 4410389..4411318 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
QN227_RS21040 (4411718) 4411718..4412698 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
QN227_RS21045 (4412927) 4412927..4413169 - 243 WP_001086388.1 protein YiiF -
QN227_RS21050 (4413387) 4413387..4413605 - 219 WP_001295676.1 CopG family transcriptional regulator -
QN227_RS21055 (4414191) 4414191..4414481 - 291 WP_000356397.1 NadS family protein Antitoxin
QN227_RS21060 (4414482) 4414482..4414793 - 312 WP_000897305.1 hypothetical protein Toxin
QN227_RS21065 (4415022) 4415022..4415930 + 909 WP_033557298.1 alpha/beta hydrolase -
QN227_RS21070 (4415994) 4415994..4416935 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
QN227_RS21075 (4416980) 4416980..4417417 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
QN227_RS21080 (4417414) 4417414..4418286 - 873 WP_000920762.1 virulence factor BrkB family protein -
QN227_RS21085 (4418280) 4418280..4418879 - 600 WP_001295269.1 glucose-1-phosphatase -
QN227_RS21090 (4418978) 4418978..4419763 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4411718..4412698 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T282910 WP_000897305.1 NZ_CP126348:c4414793-4414482 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT282910 WP_000356397.1 NZ_CP126348:c4414481-4414191 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References