Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1095351..1096078 Replicon chromosome
Accession NZ_CP126348
Organism Escherichia coli strain NN5

Toxin (Protein)


Gene name higB Uniprot ID I2UEW4
Locus tag QN227_RS05300 Protein ID WP_000547559.1
Coordinates 1095351..1095662 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QN227_RS05305 Protein ID WP_000126294.1
Coordinates 1095659..1096078 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN227_RS05270 (1090493) 1090493..1092202 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
QN227_RS05275 (1092212) 1092212..1092754 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
QN227_RS05280 (1092754) 1092754..1093521 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
QN227_RS05285 (1093518) 1093518..1093928 + 411 WP_001291922.1 formate hydrogenlyase assembly protein HycH -
QN227_RS05290 (1093921) 1093921..1094391 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
QN227_RS05295 (1094434) 1094434..1095177 + 744 WP_001341829.1 hypothetical protein -
QN227_RS05300 (1095351) 1095351..1095662 + 312 WP_000547559.1 type II toxin-antitoxin system HigB family toxin Toxin
QN227_RS05305 (1095659) 1095659..1096078 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
QN227_RS05310 (1096192) 1096192..1097616 - 1425 WP_033557656.1 6-phospho-beta-glucosidase AscB -
QN227_RS05315 (1097625) 1097625..1099082 - 1458 WP_033557644.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QN227_RS05320 (1099342) 1099342..1100352 + 1011 WP_001402444.1 DNA-binding transcriptional regulator AscG -
QN227_RS05325 (1100501) 1100501..1101028 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(24-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12450.11 Da        Isoelectric Point: 9.7249

>T282896 WP_000547559.1 NZ_CP126348:1095351-1095662 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT282896 WP_000126294.1 NZ_CP126348:1095659-1096078 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3V163

Antitoxin

Source ID Structure

References