Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 594124..594923 Replicon chromosome
Accession NZ_CP126348
Organism Escherichia coli strain NN5

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag QN227_RS02900 Protein ID WP_000347273.1
Coordinates 594124..594588 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QN227_RS02905 Protein ID WP_001307405.1
Coordinates 594588..594923 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN227_RS02870 (589125) 589125..589559 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QN227_RS02875 (589577) 589577..590455 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
QN227_RS02880 (590445) 590445..591224 - 780 WP_033557704.1 PTS N-acetylgalactosamine transporter subunit IIC -
QN227_RS02885 (591235) 591235..591708 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QN227_RS02890 (591731) 591731..593011 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QN227_RS02895 (593260) 593260..594069 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
QN227_RS02900 (594124) 594124..594588 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QN227_RS02905 (594588) 594588..594923 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QN227_RS02910 (595073) 595073..596644 - 1572 WP_033557705.1 galactarate dehydratase -
QN227_RS02915 (597019) 597019..598353 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
QN227_RS02920 (598369) 598369..599139 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 594124..605912 11788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T282893 WP_000347273.1 NZ_CP126348:c594588-594124 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT282893 WP_001307405.1 NZ_CP126348:c594923-594588 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References