Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 666374..667101 Replicon chromosome
Accession NZ_CP126343
Organism Escherichia coli strain DAX4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QN220_RS03270 Protein ID WP_000550189.1
Coordinates 666374..666688 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QN220_RS03275 Protein ID WP_000560266.1
Coordinates 666685..667101 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QN220_RS03250 (662532) 662532..663518 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
QN220_RS03255 (663597) 663597..664289 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
QN220_RS03260 (664366) 664366..664869 - 504 WP_001333820.1 M48 family metallopeptidase -
QN220_RS03265 (664954) 664954..666090 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QN220_RS03270 (666374) 666374..666688 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QN220_RS03275 (666685) 666685..667101 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QN220_RS03280 (667146) 667146..669164 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QN220_RS03285 (669590) 669590..671941 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T282848 WP_000550189.1 NZ_CP126343:666374-666688 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT282848 WP_000560266.1 NZ_CP126343:666685-667101 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References