Detailed information of TA system TA028275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3638572..3639140
• Replicon: chromosome
• Accession: NC_018645
• Organism: Desulfobacula toluolica Tol2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: TOL2_RS16595
• Protein ID: WP_041279587.1
• Coordinates: 3638862..3639140 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TOL2_RS16590
• Protein ID: WP_041279586.1
• Coordinates: 3638572..3638862 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL2_RS25005	3634447..3634611	-	165	WP_158406101.1	hypothetical protein	-
TOL2_RS16585	3635014..3636354	+	1341	WP_014958452.1	group II intron reverse transcriptase/maturase	-
TOL2_RS16590	3638572..3638862	-	291	WP_041279586.1	HigA family addiction module antidote protein	Antitoxin
TOL2_RS16595	3638862..3639140	-	279	WP_041279587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOL2_RS16600	3639514..3639729	-	216	WP_193787650.1	hypothetical protein	-
TOL2_RS16605	3639816..3640172	-	357	WP_041279588.1	DMT family protein	-
TOL2_RS16610	3640447..3640992	+	546	WP_014958456.1	hypothetical protein	-
TOL2_RS16615	3641048..3641326	-	279	WP_198408376.1	helix-turn-helix transcriptional regulator	-
TOL2_RS16620	3641331..3641663	-	333	Protein_3231	type II toxin-antitoxin system RelE/ParE family toxin	-
TOL2_RS16625	3641735..3642862	+	1128	WP_014958459.1	site-specific integrase	-
TOL2_RS16630	3642880..3643974	+	1095	WP_014958460.1	three-Cys-motif partner protein TcmP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3565501..3651268	85767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10820.50 Da
• Isoelectric Point: 10.0678

>T28275 WP_041279587.1 NC_018645:c3639140-3638862 [Desulfobacula toluolica Tol2]
MIKTFKHKGLRKFFETGKLSGIQPSHKQKLRIRLIALDTATCIEDMNTPGWRLHGLKGDRQGLWAIDVNKNWRIVFEFKD
GNAYIVDYEDYH

Nucleotide

• Length: 279 bp

>T28275 NC_018645:c3639140-3638862 [Desulfobacula toluolica Tol2]
ATGATAAAAACATTTAAACACAAAGGGCTACGTAAGTTCTTTGAAACTGGTAAGTTGTCAGGTATTCAACCCAGTCACAA
ACAAAAGCTCAGAATTCGACTAATTGCACTTGATACTGCGACATGTATAGAAGATATGAATACTCCCGGTTGGCGGCTCC
ATGGTTTAAAAGGTGATCGTCAAGGATTATGGGCGATTGATGTAAACAAAAACTGGCGTATCGTTTTTGAATTTAAAGAT
GGTAATGCCTATATAGTAGATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10570.39 Da
• Isoelectric Point: 7.9999

>AT28275 WP_041279586.1 NC_018645:c3638862-3638572 [Desulfobacula toluolica Tol2]
MAMYNPPHPGEFIKEVYLEPLSVSPRSVALLLKVSPSTFSRLIKGQSAISPAMALRLSKCLGRTPESWLLMQDSYDLWQA
KQSVDLSGIEPLAIPA

Nucleotide

• Length: 291 bp

>AT28275 NC_018645:c3638862-3638572 [Desulfobacula toluolica Tol2]
ATGGCTATGTATAACCCACCCCATCCTGGGGAATTCATTAAAGAAGTTTATTTAGAGCCTTTAAGTGTATCGCCCCGTAG
CGTTGCTTTACTGCTTAAGGTCTCACCTTCAACCTTTTCAAGGCTTATAAAAGGGCAAAGTGCAATCAGTCCAGCAATGG
CCCTACGCTTATCTAAATGCTTAGGTCGTACTCCTGAAAGTTGGCTTCTCATGCAAGACAGCTATGATCTTTGGCAGGCT
AAGCAAAGTGTTGATCTTTCTGGAATAGAGCCACTGGCAATCCCTGCTTAA