282298

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3764292..3764986 Replicon chromosome
Accession NZ_CP125731
Organism Escherichia coli strain GYB01

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QMW19_RS18295 Protein ID WP_001263489.1
Coordinates 3764292..3764690 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QMW19_RS18300 Protein ID WP_000554758.1
Coordinates 3764693..3764986 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3759880) 3759880..3759960 - 81 NuclAT_11 - -
- (3759880) 3759880..3759960 - 81 NuclAT_11 - -
- (3759880) 3759880..3759960 - 81 NuclAT_11 - -
- (3759880) 3759880..3759960 - 81 NuclAT_11 - -
QMW19_RS18270 (3760556) 3760556..3761014 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
QMW19_RS18275 (3761275) 3761275..3762732 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QMW19_RS18280 (3762789) 3762789..3763310 - 522 Protein_3575 peptide chain release factor H -
QMW19_RS18285 (3763306) 3763306..3763512 - 207 Protein_3576 RtcB family protein -
QMW19_RS18290 (3763830) 3763830..3764282 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
QMW19_RS18295 (3764292) 3764292..3764690 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QMW19_RS18300 (3764693) 3764693..3764986 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QMW19_RS18305 (3765038) 3765038..3766093 - 1056 WP_001226164.1 DNA polymerase IV -
QMW19_RS18310 (3766164) 3766164..3766949 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QMW19_RS18315 (3766921) 3766921..3768633 + 1713 Protein_3582 flagellar biosynthesis protein FlhA -
QMW19_RS18320 (3768857) 3768857..3769354 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3764292..3779572 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T282298 WP_001263489.1 NZ_CP125731:c3764690-3764292 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT282298 WP_000554758.1 NZ_CP125731:c3764986-3764693 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References