Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3534688..3535358 Replicon chromosome
Accession NZ_CP125620
Organism Mycobacterium tuberculosis strain BLR 4299 2019

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag O3Q54_RS16765 Protein ID WP_003899954.1
Coordinates 3534688..3535032 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag O3Q54_RS16770 Protein ID WP_003899955.1
Coordinates 3535029..3535358 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3Q54_RS16730 (O3Q54_16730) 3529761..3530621 + 861 WP_003416628.1 alpha/beta hydrolase -
O3Q54_RS16735 (O3Q54_16735) 3530596..3531111 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
O3Q54_RS16740 (O3Q54_16740) 3531127..3531338 + 212 Protein_3305 (R)-hydratase -
O3Q54_RS16745 (O3Q54_16745) 3531351..3531644 + 294 WP_003416635.1 hypothetical protein -
O3Q54_RS16750 (O3Q54_16750) 3531932..3533221 + 1290 WP_003906996.1 ATP-binding protein -
O3Q54_RS16755 (O3Q54_16755) 3533568..3534002 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
O3Q54_RS16760 (O3Q54_16760) 3534005..3534457 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
O3Q54_RS16765 (O3Q54_16765) 3534688..3535032 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
O3Q54_RS16770 (O3Q54_16770) 3535029..3535358 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
O3Q54_RS16775 (O3Q54_16775) 3535896..3536243 + 348 WP_003899956.1 DUF2384 domain-containing protein -
O3Q54_RS16780 (O3Q54_16780) 3536240..3536860 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
O3Q54_RS16785 (O3Q54_16785) 3537020..3538285 - 1266 WP_003902423.1 hypothetical protein -
O3Q54_RS16790 (O3Q54_16790) 3538453..3538662 + 210 WP_003416778.1 hypothetical protein -
O3Q54_RS16795 (O3Q54_16795) 3538909..3539943 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T282217 WP_003899954.1 NZ_CP125620:3534688-3535032 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT282217 WP_003899955.1 NZ_CP125620:3535029-3535358 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References