Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2247938..2248857 Replicon chromosome
Accession NZ_CP125620
Organism Mycobacterium tuberculosis strain BLR 4299 2019

Toxin (Protein)


Gene name HigB2 Uniprot ID A0A654U351
Locus tag O3Q54_RS10565 Protein ID WP_003906719.1
Coordinates 2248252..2248857 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag O3Q54_RS10560 Protein ID WP_003410124.1
Coordinates 2247938..2248243 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3Q54_RS10530 (O3Q54_10530) 2242949..2244205 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
O3Q54_RS10535 (O3Q54_10535) 2244559..2245134 + 576 WP_003410100.1 hypothetical protein -
O3Q54_RS10540 (O3Q54_10540) 2245131..2246171 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
O3Q54_RS10545 (O3Q54_10545) 2246413..2247132 + 720 WP_003410108.1 DUF433 domain-containing protein -
O3Q54_RS10550 (O3Q54_10550) 2247122..2247538 + 417 WP_003410114.1 hypothetical protein -
O3Q54_RS10555 (O3Q54_10555) 2247554..2247853 - 300 WP_003410120.1 hypothetical protein -
O3Q54_RS10560 (O3Q54_10560) 2247938..2248243 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
O3Q54_RS10565 (O3Q54_10565) 2248252..2248857 - 606 WP_003906719.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
O3Q54_RS10570 (O3Q54_10570) 2248882..2249241 - 360 WP_003410131.1 hypothetical protein -
O3Q54_RS10575 (O3Q54_10575) 2249401..2250033 - 633 WP_003913310.1 hypothetical protein -
O3Q54_RS10580 (O3Q54_10580) 2250030..2250266 - 237 WP_003410133.1 hypothetical protein -
O3Q54_RS10585 (O3Q54_10585) 2250473..2251369 - 897 WP_003906720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22716.91 Da        Isoelectric Point: 7.3073

>T282199 WP_003906719.1 NZ_CP125620:c2248857-2248252 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT282199 WP_003410124.1 NZ_CP125620:c2248243-2247938 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654U351

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References