Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 130521..131157 Replicon chromosome
Accession NZ_CP124968
Organism Agrobacterium tumefaciens strain O54/95

Toxin (Protein)


Gene name higB Uniprot ID A0A846LJX3
Locus tag G6L90_RS15130 Protein ID WP_060727225.1
Coordinates 130876..131157 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A846LMK5
Locus tag G6L90_RS15125 Protein ID WP_060727224.1
Coordinates 130521..130820 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6L90_RS15100 (G6L90_15100) 125704..126432 - 729 WP_080864439.1 ABC transporter ATP-binding protein -
G6L90_RS15105 (G6L90_15105) 126429..127337 - 909 WP_020009811.1 branched-chain amino acid ABC transporter permease -
G6L90_RS15110 (G6L90_15110) 127334..128206 - 873 WP_113076637.1 branched-chain amino acid ABC transporter permease -
G6L90_RS15115 (G6L90_15115) 128277..129491 - 1215 WP_020009809.1 amino acid ABC transporter substrate-binding protein -
G6L90_RS15120 (G6L90_15120) 129627..130499 + 873 WP_129566064.1 AraC family transcriptional regulator -
G6L90_RS15125 (G6L90_15125) 130521..130820 - 300 WP_060727224.1 HigA family addiction module antitoxin Antitoxin
G6L90_RS15130 (G6L90_15130) 130876..131157 - 282 WP_060727225.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G6L90_RS15135 (G6L90_15135) 131233..132042 - 810 WP_060727226.1 ABC transporter ATP-binding protein -
G6L90_RS15140 (G6L90_15140) 132039..133073 - 1035 WP_060727227.1 iron chelate uptake ABC transporter family permease subunit -
G6L90_RS15145 (G6L90_15145) 133076..134119 - 1044 WP_174071003.1 iron ABC transporter permease -
G6L90_RS15150 (G6L90_15150) 134116..135126 - 1011 WP_174071001.1 iron-siderophore ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(19-70)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10562.03 Da        Isoelectric Point: 8.0066

>T281862 WP_060727225.1 NZ_CP124968:c131157-130876 [Agrobacterium tumefaciens]
MIRSFKNKLAASIDDGSVKKVFPADLVRRAQQLLTILDAATTVEDLRSPPGNRLEKLSGDREGQYSIRINKQWRICFVWT
EAGPESVEITDYH

Download         Length: 282 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11127.05 Da        Isoelectric Point: 8.5148

>AT281862 WP_060727224.1 NZ_CP124968:c130820-130521 [Agrobacterium tumefaciens]
MTISKLPPIHPGEILRELYLEPLGMSAYALARHLNIPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
VAAVRLEADLANIKELEAA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A846LJX3

Antitoxin

Source ID Structure
AlphaFold DB A0A846LMK5

References