Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1231059..1231708 Replicon chromosome
Accession NC_018405
Organism Enterobacter cloacae subsp. cloacae ENHKU01

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0PLQ3
Locus tag ECENHK_RS05790 Protein ID WP_014882864.1
Coordinates 1231346..1231708 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag ECENHK_RS05785 Protein ID WP_014882863.1
Coordinates 1231059..1231358 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECENHK_RS05775 1227484..1229457 + 1974 WP_014882861.1 PhoX family phosphatase -
ECENHK_RS05780 1229560..1230948 + 1389 WP_014882862.1 phenylalanine transporter -
ECENHK_RS05785 1231059..1231358 - 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
ECENHK_RS05790 1231346..1231708 - 363 WP_014882864.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECENHK_RS05795 1231914..1233149 + 1236 WP_014882865.1 MFS transporter -
ECENHK_RS05800 1233165..1234190 + 1026 WP_014882866.1 sugar phosphate isomerase/epimerase -
ECENHK_RS05805 1234183..1235325 + 1143 WP_014882867.1 Gfo/Idh/MocA family oxidoreductase -
ECENHK_RS05810 1235401..1236372 + 972 WP_014882868.1 LacI family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13952.92 Da        Isoelectric Point: 6.7114

>T28149 WP_014882864.1 NC_018405:c1231708-1231346 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T28149 NC_018405:c1231708-1231346 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTGAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT28149 WP_014882863.1 NC_018405:c1231358-1231059 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT28149 NC_018405:c1231358-1231059 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PLQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References