Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4618314..4619041 Replicon chromosome
Accession NZ_CP124819
Organism Escherichia coli strain C325

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag QLG17_RS22950 Protein ID WP_000547563.1
Coordinates 4618314..4618625 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QLG17_RS22955 Protein ID WP_000126294.1
Coordinates 4618622..4619041 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLG17_RS22920 (4613459) 4613459..4615168 + 1710 WP_001288147.1 formate hydrogenlyase subunit HycE -
QLG17_RS22925 (4615178) 4615178..4615720 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
QLG17_RS22930 (4615720) 4615720..4616487 + 768 WP_000067400.1 formate hydrogenlyase subunit HycG -
QLG17_RS22935 (4616484) 4616484..4616894 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
QLG17_RS22940 (4616887) 4616887..4617354 + 468 WP_000132960.1 hydrogenase maturation peptidase HycI -
QLG17_RS22945 (4617397) 4617397..4618152 + 756 WP_001400787.1 hypothetical protein -
QLG17_RS22950 (4618314) 4618314..4618625 + 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
QLG17_RS22955 (4618622) 4618622..4619041 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
QLG17_RS22960 (4619155) 4619155..4620579 - 1425 WP_000110310.1 6-phospho-beta-glucosidase AscB -
QLG17_RS22965 (4620588) 4620588..4622045 - 1458 WP_001107816.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
QLG17_RS22970 (4622306) 4622306..4623316 + 1011 WP_032161993.1 DNA-binding transcriptional regulator AscG -
QLG17_RS22975 (4623465) 4623465..4623992 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T281288 WP_000547563.1 NZ_CP124819:4618314-4618625 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT281288 WP_000126294.1 NZ_CP124819:4618622-4619041 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References