Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4205692..4206419 Replicon chromosome
Accession NZ_CP124819
Organism Escherichia coli strain C325

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QLG17_RS21000 Protein ID WP_000550189.1
Coordinates 4205692..4206006 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QLG17_RS21005 Protein ID WP_052915301.1
Coordinates 4206003..4206419 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLG17_RS20980 (4201851) 4201851..4202837 - 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -
QLG17_RS20985 (4202916) 4202916..4203606 - 691 Protein_4096 vancomycin high temperature exclusion protein -
QLG17_RS20990 (4203683) 4203683..4204186 - 504 WP_001295542.1 M48 family metallopeptidase -
QLG17_RS20995 (4204271) 4204271..4205407 + 1137 WP_025380598.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QLG17_RS21000 (4205692) 4205692..4206006 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QLG17_RS21005 (4206003) 4206003..4206419 + 417 WP_052915301.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QLG17_RS21010 (4206464) 4206464..4208482 - 2019 WP_052915300.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QLG17_RS21015 (4208786) 4208786..4210330 - 1545 WP_000258466.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T281284 WP_000550189.1 NZ_CP124819:4205692-4206006 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT281284 WP_052915301.1 NZ_CP124819:4206003-4206419 [Escherichia coli]
MIAITDILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References