Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 321465..322131 Replicon chromosome
Accession NZ_CP124813
Organism Citrobacter freundii strain CF12

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag QLG15_RS01515 Protein ID WP_003847996.1
Coordinates 321814..322131 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag QLG15_RS01510 Protein ID WP_003837894.1
Coordinates 321465..321761 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLG15_RS01495 (318641) 318641..319114 - 474 WP_003023524.1 transcription elongation factor GreB -
QLG15_RS01500 (319340) 319340..320059 + 720 WP_001157751.1 two-component system response regulator OmpR -
QLG15_RS01505 (320056) 320056..321408 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
QLG15_RS01510 (321465) 321465..321761 - 297 WP_003837894.1 NadS family protein Antitoxin
QLG15_RS01515 (321814) 321814..322131 - 318 WP_003847996.1 hypothetical protein Toxin
QLG15_RS01520 (322254) 322254..323876 - 1623 WP_044701411.1 phosphoenolpyruvate carboxykinase (ATP) -
QLG15_RS01525 (324255) 324255..325973 + 1719 WP_047715944.1 DUF4153 domain-containing protein -
QLG15_RS01530 (326083) 326083..326961 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T281256 WP_003847996.1 NZ_CP124813:c322131-321814 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT281256 WP_003837894.1 NZ_CP124813:c321761-321465 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References