Detailed information of TA system TA028119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1745570..1746360
• Replicon: chromosome
• Accession: NC_018220
• Organism: Pseudomonas putida DOT-T1E

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: T1E_RS08175
• Protein ID: WP_014859916.1
• Coordinates: 1745570..1746037 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: I7C311
• Locus tag: T1E_RS08180
• Protein ID: WP_014859917.1
• Coordinates: 1746034..1746360 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
T1E_RS08155	1740733..1742262	+	1530	WP_014859913.1	efflux transporter outer membrane subunit	-
T1E_RS08160	1742259..1744436	+	2178	WP_003251716.1	FUSC family protein	-
T1E_RS08165	1744426..1744626	+	201	WP_014859914.1	DUF1656 domain-containing protein	-
T1E_RS08170	1744637..1745497	+	861	WP_014859915.1	HlyD family secretion protein	-
T1E_RS08175	1745570..1746037	-	468	WP_014859916.1	type II toxin-antitoxin system YhaV family toxin	Toxin
T1E_RS08180	1746034..1746360	-	327	WP_014859917.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
T1E_RS08185	1746453..1746899	-	447	WP_014859918.1	universal stress protein	-
T1E_RS08190	1747173..1748078	+	906	WP_014859919.1	LysR family transcriptional regulator	-
T1E_RS08195	1748214..1749755	+	1542	WP_014859920.1	MFS transporter	-
T1E_RS08200	1749784..1750845	+	1062	WP_014859921.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (17-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17998.38 Da
• Isoelectric Point: 9.7964

>T28119 WP_014859916.1 NC_018220:c1746037-1745570 [Pseudomonas putida DOT-T1E]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWVNDESSKRAYESNDDAYKVFQKMLHSGHPPDDWDQLLQEAAAD

Nucleotide

• Length: 468 bp

>T28119 NC_018220:c1746037-1745570 [Pseudomonas putida DOT-T1E]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGCGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAACGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCCACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11919.40 Da
• Isoelectric Point: 4.3722

>AT28119 WP_014859917.1 NC_018220:c1746360-1746034 [Pseudomonas putida DOT-T1E]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Nucleotide

• Length: 327 bp

>AT28119 NC_018220:c1746360-1746034 [Pseudomonas putida DOT-T1E]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Structures

Antitoxin

• AlphaFold DB: I7C311