Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 84315..85042 | Replicon | plasmid p1-KP21315 |
| Accession | NZ_CP124703 | ||
| Organism | Klebsiella pneumoniae strain KP21315 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | QJQ34_RS26495 | Protein ID | WP_011251285.1 |
| Coordinates | 84315..84626 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | QJQ34_RS26500 | Protein ID | WP_011251286.1 |
| Coordinates | 84623..85042 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QJQ34_RS26465 (QJQ34_26465) | 79506..80486 | + | 981 | WP_012569499.1 | IS5-like element ISKpn26 family transposase | - |
| QJQ34_RS26470 (QJQ34_26470) | 81032..81211 | - | 180 | Protein_94 | IS5/IS1182 family transposase | - |
| QJQ34_RS26475 (QJQ34_26475) | 81251..82789 | - | 1539 | WP_017901237.1 | IS66 family transposase | - |
| QJQ34_RS26480 (QJQ34_26480) | 82838..83185 | - | 348 | WP_032430752.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| QJQ34_RS26485 (QJQ34_26485) | 83182..83559 | - | 378 | WP_004118218.1 | transposase | - |
| QJQ34_RS26490 (QJQ34_26490) | 83673..84110 | + | 438 | Protein_98 | DDE-type integrase/transposase/recombinase | - |
| QJQ34_RS26495 (QJQ34_26495) | 84315..84626 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| QJQ34_RS26500 (QJQ34_26500) | 84623..85042 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| QJQ34_RS26505 (QJQ34_26505) | 85112..85525 | + | 414 | WP_017901337.1 | hypothetical protein | - |
| QJQ34_RS26510 (QJQ34_26510) | 85571..86539 | - | 969 | WP_074168518.1 | IS5-like element IS903B family transposase | - |
| QJQ34_RS26515 (QJQ34_26515) | 86834..87859 | - | 1026 | WP_282499563.1 | IS110 family transposase | - |
| QJQ34_RS26520 (QJQ34_26520) | 88017..88283 | + | 267 | WP_017900868.1 | hypothetical protein | - |
| QJQ34_RS26525 (QJQ34_26525) | 88397..88932 | - | 536 | Protein_105 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | catA1 / tet(D) / blaSHV-12 | - | 1..254179 | 254179 | |
| - | inside | IScluster/Tn | - | - | 79506..88791 | 9285 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T281119 WP_011251285.1 NZ_CP124703:84315-84626 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT281119 WP_011251286.1 NZ_CP124703:84623-85042 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|