Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6059877..6060472 Replicon chromosome
Accession NZ_CP124649
Organism Pseudomonas aeruginosa strain 2020CK-00218

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag P7I80_RS28850 Protein ID WP_003113526.1
Coordinates 6060194..6060472 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag P7I80_RS28845 Protein ID WP_003111575.1
Coordinates 6059877..6060182 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7I80_RS28810 (P7I80_28810) 6055017..6055865 + 849 WP_023096155.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
P7I80_RS28820 (P7I80_28820) 6056032..6056973 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
P7I80_RS28825 (P7I80_28825) 6057090..6057704 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
P7I80_RS28830 (P7I80_28830) 6057746..6058330 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
P7I80_RS28835 (P7I80_28835) 6058371..6059471 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
P7I80_RS28845 (P7I80_28845) 6059877..6060182 - 306 WP_003111575.1 HigA family addiction module antitoxin Antitoxin
P7I80_RS28850 (P7I80_28850) 6060194..6060472 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P7I80_RS28855 (P7I80_28855) 6060525..6060653 - 129 Protein_5702 integrase -
P7I80_RS28860 (P7I80_28860) 6060801..6063029 + 2229 WP_021264080.1 TonB-dependent receptor -
P7I80_RS28865 (P7I80_28865) 6063099..6063746 - 648 WP_003095021.1 carbonate dehydratase -
P7I80_RS28870 (P7I80_28870) 6063808..6065046 - 1239 WP_014603324.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T280950 WP_003113526.1 NZ_CP124649:c6060472-6060194 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT280950 WP_003111575.1 NZ_CP124649:c6060182-6059877 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References