Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4610452..4611131 Replicon chromosome
Accession NZ_CP124517
Organism Escherichia coli strain AVS0343

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJB09_RS22475 Protein ID WP_000057523.1
Coordinates 4610452..4610754 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJB09_RS22480 Protein ID WP_000806442.1
Coordinates 4610790..4611131 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB09_RS22450 (4605828) 4605828..4607480 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QJB09_RS22455 (4607518) 4607518..4608021 - 504 WP_000667000.1 hypothetical protein -
QJB09_RS22460 (4608018) 4608018..4608818 - 801 WP_000439798.1 hypothetical protein -
QJB09_RS22465 (4608842) 4608842..4609321 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJB09_RS22470 (4609525) 4609525..4610319 - 795 WP_000365147.1 TraB/GumN family protein -
QJB09_RS22475 (4610452) 4610452..4610754 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJB09_RS22480 (4610790) 4610790..4611131 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJB09_RS22485 (4611189) 4611189..4613693 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJB09_RS22490 (4613955) 4613955..4614887 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280803 WP_000057523.1 NZ_CP124517:4610452-4610754 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280803 WP_000806442.1 NZ_CP124517:4610790-4611131 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References