Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3524173..3524852 Replicon chromosome
Accession NZ_CP124492
Organism Escherichia coli strain AVS0225

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJA99_RS17330 Protein ID WP_000057523.1
Coordinates 3524550..3524852 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJA99_RS17325 Protein ID WP_000806442.1
Coordinates 3524173..3524514 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJA99_RS17315 (3520417) 3520417..3521349 - 933 WP_000883041.1 glutaminase A -
QJA99_RS17320 (3521611) 3521611..3524115 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJA99_RS17325 (3524173) 3524173..3524514 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJA99_RS17330 (3524550) 3524550..3524852 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJA99_RS17335 (3524985) 3524985..3525779 + 795 WP_000365147.1 TraB/GumN family protein -
QJA99_RS17340 (3525983) 3525983..3526462 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJA99_RS17345 (3526486) 3526486..3527286 + 801 WP_000439798.1 hypothetical protein -
QJA99_RS17350 (3527283) 3527283..3527786 + 504 WP_000667000.1 hypothetical protein -
QJA99_RS17355 (3527824) 3527824..3529476 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280568 WP_000057523.1 NZ_CP124492:c3524852-3524550 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280568 WP_000806442.1 NZ_CP124492:c3524514-3524173 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References