Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3466163..3466842 Replicon chromosome
Accession NZ_CP124490
Organism Escherichia coli strain AVS0456

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP91_RS16870 Protein ID WP_000057523.1
Coordinates 3466540..3466842 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP91_RS16865 Protein ID WP_000806442.1
Coordinates 3466163..3466504 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP91_RS16855 (3462407) 3462407..3463339 - 933 WP_000883041.1 glutaminase A -
QJP91_RS16860 (3463601) 3463601..3466105 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP91_RS16865 (3466163) 3466163..3466504 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP91_RS16870 (3466540) 3466540..3466842 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP91_RS16875 (3466975) 3466975..3467769 + 795 WP_000365147.1 TraB/GumN family protein -
QJP91_RS16880 (3467973) 3467973..3468452 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP91_RS16885 (3468476) 3468476..3469276 + 801 WP_000439798.1 hypothetical protein -
QJP91_RS16890 (3469273) 3469273..3469776 + 504 WP_000667000.1 hypothetical protein -
QJP91_RS16895 (3469814) 3469814..3471466 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280548 WP_000057523.1 NZ_CP124490:c3466842-3466540 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280548 WP_000806442.1 NZ_CP124490:c3466504-3466163 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References