Detailed information of TA system TA028054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2870571..2871229
• Replicon: chromosome
• Accession: NC_018142
• Organism: Propionibacterium propionicum F0230a

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A4P8ZSU4
• Locus tag: HMPREF9154_RS12845
• Protein ID: WP_014847604.1
• Coordinates: 2870571..2870930 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: HMPREF9154_RS12850
• Protein ID: WP_041696711.1
• Coordinates: 2870930..2871229 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF9154_RS12825	2865782..2866081	-	300	WP_014847601.1	HigA family addiction module antidote protein	-
HMPREF9154_RS12830	2866092..2866373	-	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HMPREF9154_RS12835	2866678..2867979	+	1302	WP_014847603.1	hypothetical protein	-
HMPREF9154_RS15785	2867976..2869775	+	1800	WP_051015059.1	tetratricopeptide repeat protein	-
HMPREF9154_RS12845	2870571..2870930	+	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HMPREF9154_RS12850	2870930..2871229	+	300	WP_041696711.1	helix-turn-helix transcriptional regulator	Antitoxin
HMPREF9154_RS12855	2871614..2872369	-	756	WP_014847606.1	transposase	-
HMPREF9154_RS12860	2872942..2873223	+	282	WP_041696713.1	helix-turn-helix domain-containing protein	-
HMPREF9154_RS12865	2873525..2874598	-	1074	WP_014847607.1	restriction endonuclease, SacI family	-
HMPREF9154_RS12870	2874654..2875811	-	1158	WP_041696714.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 10.7132

>T28054 WP_014847604.1 NC_018142:2870571-2870930 [Arachnia propionica F0230a]
MWEVDVTLIADWLRSLDQTSYEQVIAALEVLRDRGLSLGRPLVDTITASRHRNMKELRPGSIGRSEVRILFAFDPKRMAI
LLIAGDKAGSWTRWYKRNVPLADDLFDKHIRALRKRPEK

Nucleotide

• Length: 360 bp

>T28054 NC_018142:2870571-2870930 [Arachnia propionica F0230a]
ATGTGGGAGGTAGACGTCACGTTGATCGCTGACTGGCTCAGGTCACTGGACCAAACCTCCTACGAACAGGTCATCGCTGC
GCTGGAGGTGCTTCGCGATCGTGGTCTTTCACTCGGGCGTCCTCTCGTTGACACCATCACCGCTTCACGGCATCGCAACA
TGAAAGAGCTTCGCCCCGGCTCGATAGGGCGATCCGAGGTTCGCATCCTGTTCGCCTTTGACCCCAAGCGCATGGCGATC
TTGCTGATCGCGGGCGACAAGGCTGGTAGCTGGACTCGGTGGTACAAGAGGAACGTCCCACTGGCCGATGATCTGTTCGA
CAAACATATCCGCGCATTGAGGAAGAGACCAGAAAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11031.61 Da
• Isoelectric Point: 7.2036

>AT28054 WP_041696711.1 NC_018142:2870930-2871229 [Arachnia propionica F0230a]
MTIDDILAERSVDRSQVEAHKARMLAEVRAYRLRELRENAGLTQAQLAERIGVGQRQISKIEHGDLDTAKIGTIRRYLEA
IGGSLSLEYVAGDLKVVIN

Nucleotide

• Length: 300 bp

>AT28054 NC_018142:2870930-2871229 [Arachnia propionica F0230a]
ATGACAATTGACGATATTCTCGCCGAACGCTCCGTGGACCGCAGCCAAGTCGAGGCACACAAAGCCCGGATGCTCGCAGA
AGTTCGCGCCTATCGACTTCGAGAGCTCCGCGAAAATGCTGGATTGACGCAGGCGCAGTTGGCCGAACGCATCGGCGTCG
GGCAGCGTCAAATCTCCAAGATCGAGCACGGCGACCTAGACACCGCGAAGATCGGAACGATCCGCCGCTACCTCGAGGCC
ATCGGCGGCAGCCTCTCACTCGAGTACGTCGCAGGAGACCTCAAAGTCGTGATCAATTGA

Structures

Toxin

• AlphaFold DB: A0A4P8ZSU4