Detailed information of TA system TA028053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2865782..2866373
• Replicon: chromosome
• Accession: NC_018142
• Organism: Propionibacterium propionicum F0230a

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A4V1GCM2
• Locus tag: HMPREF9154_RS12830
• Protein ID: WP_014847602.1
• Coordinates: 2866092..2866373 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HMPREF9154_RS12825
• Protein ID: WP_014847601.1
• Coordinates: 2865782..2866081 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMPREF9154_RS16480	2861453..2861677	-	225	WP_014847597.1	toxin-antitoxin system toxin component PIN family	-
HMPREF9154_RS12810	2861846..2863075	+	1230	WP_014847598.1	ATP-binding protein	-
HMPREF9154_RS12815	2863197..2864030	-	834	WP_157682480.1	DUF4839 domain-containing protein	-
HMPREF9154_RS17055	2864691..2864843	-	153	WP_014847599.1	hypothetical protein	-
HMPREF9154_RS12820	2865228..2865737	-	510	WP_014847600.1	GNAT family N-acetyltransferase	-
HMPREF9154_RS12825	2865782..2866081	-	300	WP_014847601.1	HigA family addiction module antidote protein	Antitoxin
HMPREF9154_RS12830	2866092..2866373	-	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HMPREF9154_RS12835	2866678..2867979	+	1302	WP_014847603.1	hypothetical protein	-
HMPREF9154_RS15785	2867976..2869775	+	1800	WP_051015059.1	tetratricopeptide repeat protein	-
HMPREF9154_RS12845	2870571..2870930	+	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HMPREF9154_RS12850	2870930..2871229	+	300	WP_041696711.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10976.73 Da
• Isoelectric Point: 10.0040

>T28053 WP_014847602.1 NC_018142:c2866373-2866092 [Arachnia propionica F0230a]
MIVSFADRDAERLFQRQRVKRLDPRLHRKALIKLWLLDAVHELDELRVPPGNRLEALKGARIGQHSIRINDQWRICFVWT
PAGPSEVEIVDYH

Nucleotide

• Length: 282 bp

>T28053 NC_018142:c2866373-2866092 [Arachnia propionica F0230a]
ATGATCGTCTCCTTCGCCGATCGCGACGCTGAGCGACTGTTCCAAAGGCAGCGCGTCAAGCGCCTTGATCCTCGTCTCCA
CAGGAAGGCGCTCATCAAGTTGTGGTTGTTGGATGCCGTCCACGAACTCGACGAGCTTCGAGTGCCGCCGGGTAACCGGC
TGGAGGCGCTCAAGGGAGCCCGAATCGGTCAGCACAGCATCAGGATCAATGACCAGTGGCGGATATGTTTCGTGTGGACC
CCGGCGGGCCCGAGCGAAGTTGAGATCGTGGACTACCATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11197.96 Da
• Isoelectric Point: 7.4040

>AT28053 WP_014847601.1 NC_018142:c2866081-2865782 [Arachnia propionica F0230a]
MDNKMAPVHPGEILVEEFLNPMGVTQHRLAISIGVPPRRINEIVHGKRRITADTALRLGRFFGMNPQFWVNLQSHYDLEI
GKDALGDELDKIQPLRSVA

Nucleotide

• Length: 300 bp

>AT28053 NC_018142:c2866081-2865782 [Arachnia propionica F0230a]
ATGGACAACAAAATGGCTCCGGTTCATCCAGGAGAAATTCTCGTGGAGGAGTTCCTGAATCCGATGGGCGTTACTCAGCA
TCGGCTCGCGATCTCCATTGGGGTGCCGCCCCGCCGAATCAACGAGATTGTGCACGGCAAGCGTCGGATCACGGCTGACA
CAGCATTGCGGCTGGGACGCTTCTTCGGAATGAATCCGCAATTCTGGGTCAACTTGCAATCGCACTACGATCTCGAAATC
GGGAAGGATGCACTGGGCGATGAGCTGGACAAGATTCAGCCTCTGAGATCTGTCGCATGA

Structures

Toxin

• AlphaFold DB: A0A4V1GCM2