Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3593960..3594639 Replicon chromosome
Accession NZ_CP124471
Organism Escherichia coli strain AVS0973

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QJP95_RS17735 Protein ID WP_000057523.1
Coordinates 3594337..3594639 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QJP95_RS17730 Protein ID WP_000806442.1
Coordinates 3593960..3594301 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP95_RS17720 (3590204) 3590204..3591136 - 933 WP_000883041.1 glutaminase A -
QJP95_RS17725 (3591398) 3591398..3593902 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QJP95_RS17730 (3593960) 3593960..3594301 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QJP95_RS17735 (3594337) 3594337..3594639 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QJP95_RS17740 (3594772) 3594772..3595566 + 795 WP_000365147.1 TraB/GumN family protein -
QJP95_RS17745 (3595770) 3595770..3596249 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QJP95_RS17750 (3596417) 3596417..3597073 + 657 WP_015674862.1 hypothetical protein -
QJP95_RS17755 (3597070) 3597070..3597573 + 504 WP_000667000.1 hypothetical protein -
QJP95_RS17760 (3597611) 3597611..3599263 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T280413 WP_000057523.1 NZ_CP124471:c3594639-3594337 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT280413 WP_000806442.1 NZ_CP124471:c3594301-3593960 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References