Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 650210..650937 Replicon chromosome
Accession NZ_CP124467
Organism Escherichia coli strain AVS0747

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QJB08_RS03235 Protein ID WP_000550189.1
Coordinates 650210..650524 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QJB08_RS03240 Protein ID WP_000560269.1
Coordinates 650521..650937 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB08_RS03210 (645645) 645645..646907 - 1263 WP_000608644.1 IS1380-like element ISEcp1 family transposase -
QJB08_RS03215 (647096) 647096..647353 - 258 Protein_630 Gfo/Idh/MocA family oxidoreductase -
QJB08_RS03220 (647432) 647432..648124 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
QJB08_RS03225 (648201) 648201..648704 - 504 WP_001295542.1 M48 family metallopeptidase -
QJB08_RS03230 (648789) 648789..649925 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QJB08_RS03235 (650210) 650210..650524 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QJB08_RS03240 (650521) 650521..650937 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QJB08_RS03245 (650982) 650982..653000 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QJB08_RS03250 (653226) 653226..655577 - 2352 WP_078232978.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn blaCTX-M-1 - 644482..646907 2425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T280383 WP_000550189.1 NZ_CP124467:650210-650524 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT280383 WP_000560269.1 NZ_CP124467:650521-650937 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References