Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5993555..5994205 Replicon chromosome
Accession NC_018106
Organism Klebsiella oxytoca E718

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag A225_RS28010 Protein ID WP_014227314.1
Coordinates 5993864..5994205 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag A225_RS28005 Protein ID WP_014227313.1
Coordinates 5993555..5993854 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A225_RS27985 5989440..5990159 - 720 WP_014839839.1 phosphonate utilization transcriptional regulator PhnR -
A225_RS27990 5990300..5991403 + 1104 WP_042934427.1 2-aminoethylphosphonate--pyruvate transaminase -
A225_RS27995 5991413..5992222 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
A225_RS28000 5992255..5993436 - 1182 WP_014839841.1 purine ribonucleoside efflux pump NepI -
A225_RS28005 5993555..5993854 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
A225_RS28010 5993864..5994205 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A225_RS28015 5994456..5994908 - 453 WP_014839842.1 DUF1198 domain-containing protein -
A225_RS28020 5995086..5996477 - 1392 WP_004107098.1 hexose-6-phosphate:phosphate antiporter -
A225_RS28025 5996623..5997942 - 1320 WP_014227316.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T28036 WP_014227314.1 NC_018106:c5994205-5993864 [Klebsiella michiganensis E718]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T28036 NC_018106:c5994205-5993864 [Klebsiella michiganensis E718]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT28036 WP_014227313.1 NC_018106:c5993854-5993555 [Klebsiella michiganensis E718]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT28036 NC_018106:c5993854-5993555 [Klebsiella michiganensis E718]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References